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Seasonal dynamics of mats composed by green alga Zygnema (Zygnematophyceae)
Trumhová, Kateřina
The mats formed by Zygnema spp. are well known and frequently found in streams, puddles, seepages, and ponds. Owing to the low and inconsistent water quantity of these biotopes, algae are periodically subjected to stresses like desiccation, freezing, or strong solar radiation. Recent studies have revealed that Zygnema survives various stresses in pre-akinetes, modified resistant vegetative cells. However, earlier experiments mostly aimed at osmotic stress and desiccation. Moreover, the mats formed by these algae have seasonal dynamics that have been observed several times, not only in temperate conditions but also in the Arctic. Field observations revealed that most of the biomass is periodically lost, and new mats are formed every year. Simultaneously, we know little about the genetic composition of the mats and how it changes over time; previous work has focused mainly on the mats as an entirety. Therefore, this work mainly aimed to subject the mats formed by the algae Zygnema spp. to long-term observation and subsequent experiments. We selected a region in the Austrian Alps, which we visited several times a year, and sampled two sites with different water regimes. Further research in collaboration with colleagues occurred in the Arctic. Zygnema spp. strains were subsequently isolated from the...
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Seasonal dynamics of mats composed by green alga Zygnema (Zygnematophyceae)
Trumhová, Kateřina ; Pichrtová, Martina (advisor) ; Remias, Daniel (referee) ; Žárský, Jakub (referee)
The mats formed by Zygnema spp. are well known and frequently found in streams, puddles, seepages, and ponds. Owing to the low and inconsistent water quantity of these biotopes, algae are periodically subjected to stresses like desiccation, freezing, or strong solar radiation. Recent studies have revealed that Zygnema survives various stresses in pre-akinetes, modified resistant vegetative cells. However, earlier experiments mostly aimed at osmotic stress and desiccation. Moreover, the mats formed by these algae have seasonal dynamics that have been observed several times, not only in temperate conditions but also in the Arctic. Field observations revealed that most of the biomass is periodically lost, and new mats are formed every year. Simultaneously, we know little about the genetic composition of the mats and how it changes over time; previous work has focused mainly on the mats as an entirety. Therefore, this work mainly aimed to subject the mats formed by the algae Zygnema spp. to long-term observation and subsequent experiments. We selected a region in the Austrian Alps, which we visited several times a year, and sampled two sites with different water regimes. Further research in collaboration with colleagues occurred in the Arctic. Zygnema spp. strains were subsequently isolated from the...
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The effect of desiccation on streptophyte algae - mechanisms of stress resistance
Pošmourný, Martin ; Pichrtová, Martina (advisor) ; Vosolsobě, Stanislav (referee)
In this thesis I dealt streptophyta algae resistance against desiccation. Even though the area previously devoted only a few people. Considerable amount of work in recent years has been published on the subject. They were found interesting information and discovered new facts. Research continues on and on, and it would be useful to look at what was observed. I believe that understanding this phenomenon is the key to understanding some of the events in the evolution of nature and realizing how tough life can be on the very border of its possible occurrence. I tried to sort out the current knowledge about the mechanisms of stress resistance streptophyta algae and hope that I obtained an overview will help me understand better this issues. So far, it has been observed several approaches to defend against drying. Preventing drying, adaptation to water shortage and tolerance to desiccation. Among the preventive methods of defense include creating clusters of cells, multi-layered mats or secretion mucilage. As an adaptation to the lack of water algae evolved more complex answers in the form of changes in ultrastructure, or regulation of physiological processes. Klebsormidium is capable of half an hour to start the production of significant quantities callose and incorporate it into the cell wall. This...
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Differences in the level of aspiration and stress resistance of women floorball players Tatran Střešovice
Zabavská, Barbora ; Janák, Vladimír (advisor) ; Běhanová, Michaela (referee)
Title: Differences in the level of aspiration and stress resistance in female floorball players of Tatran Střešovice Objectives: The main aim of this research is to contribute to the search for a relation between the level of aspirations and performance in athletes. Furthermore, whether there is a difference in stress resistance in female floorball players of different ages. The general aim is to contribute to the current knowledge base in this area with new partial results based on the implementation of my own research investigation. Methods: The TATSO test was used for this research. The author of this version of the test is Janák, who modified the original test by Hošek from 1979 into its current form. The test measures the level of aspiration, resistance to stress and psychomotor tempo. The test is suitable for group investigations, but personal presence is required (it cannot be distributed electronically). It is a pencil-and-paper type of performance test. Results: I found that the older group of female floorball players had higher aspirations compared to the younger group, so the first hypothesis was confirmed. The second hypothesis, which states that the older group of female floorball players have higher load resistance, was not confirmed, as my research showed that both groups have the...
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Differences in the level of aspiration and stress resistence of women floorball players Tatran Střešovice
Zabavská, Barbora ; Janák, Vladimír (advisor) ; Běhanová, Michaela (referee)
Title: Differences in the level of aspiration and stress resistance in female floorball players of Tatran Střešovice Objectives: The main aim of this research is to contribute to the search for a relation between the level of aspirations and performance in athletes. Furthermore, whether there is a difference in stress resistance in female floorball players of different ages. The general aim is to contribute to the current knowledge base in this area with new partial results based on the implementation of my own research investigation. Methods: The TATSO test was used for this research. The author of this version of the test is Janák, who modified the original test by Hošek from 1979 into its current form. The test measures the level of aspiration, resistance to stress and psychomotor tempo. The test is suitable for group investigations, but personal presence is required (it cannot be distributed electronically). It is a pencil-and-paper type of performance test. Results: I found that the older group of female floorball players had higher aspirations compared to the younger group, so the first hypothesis was confirmed. The second hypothesis, which states that the older group of female floorball players have higher load resistance, was not confirmed, as my research showed that both groups have the...
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The effect of desiccation on streptophyte algae - mechanisms of stress resistance
Pošmourný, Martin ; Pichrtová, Martina (advisor) ; Vosolsobě, Stanislav (referee)
In this thesis I dealt streptophyta algae resistance against desiccation. Even though the area previously devoted only a few people. Considerable amount of work in recent years has been published on the subject. They were found interesting information and discovered new facts. Research continues on and on, and it would be useful to look at what was observed. I believe that understanding this phenomenon is the key to understanding some of the events in the evolution of nature and realizing how tough life can be on the very border of its possible occurrence. I tried to sort out the current knowledge about the mechanisms of stress resistance streptophyta algae and hope that I obtained an overview will help me understand better this issues. So far, it has been observed several approaches to defend against drying. Preventing drying, adaptation to water shortage and tolerance to desiccation. Among the preventive methods of defense include creating clusters of cells, multi-layered mats or secretion mucilage. As an adaptation to the lack of water algae evolved more complex answers in the form of changes in ultrastructure, or regulation of physiological processes. Klebsormidium is capable of half an hour to start the production of significant quantities callose and incorporate it into the cell wall. This...
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Genetically modified plants in relation to solving the issue of global climate changes
Nedělová, Jana ; Opatrný, Zdeněk (advisor) ; Drda Morávková, Alena (referee)
The theoretical part of the diploma thesis summarizes basic facts related to the global climate change and provides up-to-date knowledge on the issue of genetically modified plants more resistant to stress factors of environment, including specific examples of strategies regarding preparation of genetically modified plants resistant particularly to abiotic stress. Although genetically modified plants and global climate changes belong to very important and current issue which public and foremost young generation should be sufficiently aware of, contemporary biology books and high school framework educational program pay to the issue very little attention. Therefore, the goal of this thesis is not only to summarize the basic facts and current knowledge regarding this issue but mainly to handle the issue in didactic level in the form of activating learning tasks. There were 28 learning tasks created in the thesis that gradually in unconventional way familiarize students with the issue of genetically modified plants and with the impacts of climate changes. Students must actively acquire information from accompanying materials to address the tasks properly, think critically over them, utilizing acquired knowledge and experience from the past to some extent. Fourteen tasks were chosen from the file created which...
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Functional studies of Ser/Thr protein kinases and phosphatases of Pseudomonas aeruginosa
Goldová, Jana ; Branny, Pavel (advisor) ; Nešvera, Jan (referee) ; Španová, Alena (referee)
Reversible protein phosphorylation is considered the universal language for intracellular communication in all living organisms. This process, catalysed by protein kinases and phosphatases, enables the translation of extracellular signals into cellular responses and also allows for adaptation to a constantly changing environment. In recent years, a number of bacterial eukaryotic-type Ser/Thr protein kinases and phosphatases have been identified. However, their precise functions and substrates are not yet well defined. The genome of opportunistic human pathogen Pseudomonas aeruginosa contains at least five genes encoding putative eukaryotic-type Ser/Thr protein kinases and phosphatases. In the first part of this study, we have attempted to establish the role of Ser/Thr protein kinase PpkA and phosphatase PppA, which belong to type VI secretion system H1-T6SS. Double mutant strain ∆pppA-ppkA was prepared in P. aeruginosa PAO1 background. Phenotypic studies revealed that the mutant grew slower than the wild-type strain in minimal media and exhibited reduced secretion of pigment pyocyanin. In addition, the mutant had altered sensitivity to oxidative and hyperosmotic stress conditions. Consequently, mutant cells had an impaired ability to survive in murine macrophages and an attenuated virulence in the...
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